human epidermal melanocyte cell line pig1 Search Results


99
ATCC human epidermal melanocyte cell line pig1
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Human Epidermal Melanocyte Cell Line Pig1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher 100 u/ml penicillin
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
100 U/Ml Penicillin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher fetal bovine serum (fbs
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Fetal Bovine Serum (Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher human melanocyte growth supplement
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Human Melanocyte Growth Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher medium 254
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Medium 254, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher human melanocyte growth supplements
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Human Melanocyte Growth Supplements, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher m154/hmgs medium
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
M154/Hmgs Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a375  (ATCC)
99
ATCC a375
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
ATCC yumm1 7
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Yumm1 7, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
ATCC sk mel 28
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Sk Mel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC human melanoma cell lines sk mel 5
The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in <t>melanocytes</t> and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & <t>PIG1</t> as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Human Melanoma Cell Lines Sk Mel 5, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in melanocytes and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & PIG1 as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.

Journal: Frontiers in Oncology

Article Title: Long Non-Coding RNA CD27-AS1-208 Facilitates Melanoma Progression by Activating STAT3 Pathway

doi: 10.3389/fonc.2021.818178

Figure Lengend Snippet: The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in melanocytes and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & PIG1 as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.

Article Snippet: The immortalized normal human epidermal melanocyte cell line PIG1 was a gift from Dr. Caroline Le Poole, Loyola University Chicago, Maywood, IL ( ).WM793B, WM35, A375, WM-266, A2058, 451Lu, Hs294T, 1205Lu, SK-MEL-1 and SK-MEL-5 were purchased from American Type Culture Collection (ATCC) in 2014.

Techniques: Expressing, Biomarker Discovery, Immunofluorescence, Staining, Control